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Archiver > GENEALOGY-DNA > 2004-05 > 1083583431


From: "Debby Reagan" <>
Subject: Re: [DNA] FTDNA read the wrong strand on an HVR2 mtDNA test
Date: Mon, 3 May 2004 07:23:51 -0400 (Eastern Daylight Time)
References: <410-2200451364313934@earthlink.net>


It's not that they read the wrong strand.
Or did anything wrong in fact.
Ann has described this and it's in our list's
archives. But it is as you say.....
G=A and C=T..... just switch them whenever
you see the other strand's markers, to be
like the others. One company checks one
of the strands, another might test the other.
Nothing wrong about it, just different.

Warm Regards from Maine,
Debby Reagan


-------Original Message-------

From:
Date: Monday, May 03, 2004 02:45:29
To:
Subject: [DNA] FTDNA read the wrong strand on an HVR2 mtDNA test

Hi,

Right now, I have about two dozen low resolution HVR2 mtDNA matches with
FTDNA and zero high-resolution
matches.

But I've been asking the other low-res matches if they did HVR2 (and also
looked in Charles's log). Well, there is one
person who I at first thought was very different from me as well as from the
rest of the bunch is actually quite close to me - maybe even a
high-resolution match.

To explain, I forward the e-mail I just sent to a relative of the match (who
has the same maternal ancestry). I deleted the e-mail address.

Cecelia Clancy

Why Wait? Move to EarthLink.


----- Original Message -----
From: Cecelia Clancy
To:
Sent: 5/3/2004 2:37:08 AM
Subject: FTDNA read Betty's wrong strand - she and I are actually quite
close


Hi,

So far, I have the data for six people in our pack. I wrote to several more
today and don't have their results yet.

When I first analyzed the six I have so far, at first I thought that your
Betty's line separated from the rest of the pack in very ancient times. And
then at some later but still quite early point (but much later than your
Betty's), mine separated. The remaining four are more closely related and
two are exact HVR2 matches. And I thought that even though I was distant
from those remaining four, your Betty was way more "ancient" than me.

But then I noticed that for HVR2, most of Betty's values were the opposite
of mine. For example, mine is 73G and hers is 73A. I should also mention
that the DNA molecule is two strands and when they machine read the sequence
they are reading off only one strand. The other strand will have different,
but predictable readings. For example, wherever one strand has a G, the
other will have an A. Similarly, where one has a T, the other will have a C.
I noticed that for most of the values, your Betty's had the complimentary
value to mine. So I suspect that perhaps FTDNA read the wrong strand. If so,
then your Betty and I are very similar. We are not certain matches only on
three values. But since the first two (309.1 and 315.1) of these are
insertion mutations instead of point mutations, the fact that FTDNA might
have been reading the wrong strand might have thrown the computer off enough
that it did not recognize it was on an insertion and perhaps the nucleotides
it did read in !
that region tricked the computer into "thinking" that some standard value
was there. If 309.1 is off, then 315.1 might also be off. So might the point
mutation of 489 be off.

And then I checked the big mtDNA concordance. So far (i.e. 1998), not a
single person in the world has been discovered to by 73G or 150C. When I saw
that, I knew they read the wrong strand.
So, here are the values once again:

Betty's original (wrong strand) --- 73A, 150C, 195T, 263A, 295C, 309.1C, 315
1C, 489T
Betty's "corrected" ------------------- 73G, 150T, 195C, 263G, 295T, 309.1?,
315.1?, 489?
Mine -------------------------------------- 73G, 150T, 195C, 263G, 295T, 309
1C, 315.1C, 489C


For Betty's HVR1 values, they probably read the correct strand, otherwise
they probably would have had no idea which haplogroup to assign her. But
they put her in J, so they probably read the right strand for the HVR1 part
of the test.

Take Care,
Cecelia

Cecelia Clancy

Why Wait? Move to EarthLink.


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