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Archiver > GENEALOGY-DNA > 2005-07 > 1120234491


From: Doug McDonald <>
Subject: Re: [DNA] Back to Hammer's Paper
Date: Fri, 01 Jul 2005 11:14:51 -0500
References: <000a01c57e40$b2aa9e60$5a579045@Ken1> <42C54B9F.80601@scs.uiuc.edu> <000601c57e46$620b5ed0$5a579045@Ken1> <42C5516B.5010208@fuzzo.com> <003d01c57e51$0a4ebbf0$5a579045@Ken1>
In-Reply-To: <003d01c57e51$0a4ebbf0$5a579045@Ken1>


Ken Nordtvedt wrote:

> The dna soup from which they make their measurements probably contains
> strands which run both ways. Given the comment by Doug that maybe the wrong
> strands could have been read, I asked "how"? How do they technically go
> about reading the "correct" or conventional strands so different papers and
> labs are consistent with each other?


The wrong strands simply cannot be read. I was implying that perhaps
they used the complementary and reversed primers, so that the DNA
produced that went into the sequencer was the opposite strand. They
could also be using a non-dequencing method, and again just used the
reverse and complementary binding DNA.

Doug McDonald

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