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Archiver > GENEALOGY-DNA > 2005-07 > 1120256708


From: "David M. Lawrence" <>
Subject: Re: [DNA] Back to Hammer's Paper
Date: Fri, 01 Jul 2005 18:25:08 -0400
References: <000a01c57e40$b2aa9e60$5a579045@Ken1> <42C54B9F.80601@scs.uiuc.edu> <000601c57e46$620b5ed0$5a579045@Ken1> <42C5516B.5010208@fuzzo.com> <003d01c57e51$0a4ebbf0$5a579045@Ken1> <42C56482.5000401@fuzzo.com> <004701c57e7f$75b43d60$5a579045@Ken1> <42C5B265.6030604@fuzzo.com> <007d01c57e84$0fc0bec0$5a579045@Ken1>
In-Reply-To: <007d01c57e84$0fc0bec0$5a579045@Ken1>


I don't work at any lab. I don't even know which lab you're referring
to. I'm a biologist applying first principles in a common-sense manner.
The Y-chromosome, by convention, has a top -- an upper arm -- and a
bottom -- a lower arm. You would read the sequence of DNA from the top
of the upper arm to the bottom of the lower arm. Loci -- addresses of
genes -- will be numbered accordingly, and will refer to ONE strand,
either the 5' to 3' strand or the 3' to 5' strand. I'm assuming 3' to
5', since DNA polymerase and RNA polymerase read the template strand in
that direction, but I don't know for sure. ALL LABS, HOWEVER, WILL
FOLLOW THE SAME CONVENTION. The enzymes don't have a choice, neither do
the labs.

Dave

Ken Nordtvedt wrote:
> I'm not sure what to make of a triple "they know it" without explanation why
> you are so sure? Do you perchance work at the lab in question?
>
> The whole process of receiving a report involves the lab work, the sending
> of some numbers or letters or graphs to persons who records these, to a
> transcription of this information into a report that you are positive or
> negative for the SNP. In light of your last sentence, my reported "T" then
> is quite strange --- neither ancestral or derived by the academic paper
> standard, yet it "always refers to strand A..." as you say.
>
>
> ----- Original Message -----
> From: "David M. Lawrence" <>
> To: <>
> Sent: Friday, July 01, 2005 3:15 PM
> Subject: Re: [DNA] Back to Hammer's Paper
>
>
>
>>Yes, I've been trying to say they know which strand they are reading.
>>They know which direction they are reading. They know it, they know it,
>>they know it.
>>
>>A mutation at P40 will have an effect on the complementary strand --
>>that's a given considering the structure of the molecule. But the
>>mutation always refers to strand A, not strand B.
>>
>>OK?
>>
>>Dave
>>
>
>
>
>
> ==============================
> Search the US Census Collection. Over 140 million records added in the
> last 12 months. Largest online collection in the world. Learn more: http://www.ancestry.com/s13965/rd.ashx
>


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