GENEALOGY-DNA-L ArchivesArchiver > GENEALOGY-DNA > 2005-11 > 1131910015
From: David Faux <>
Subject: Re: [DNA] YSTR Marker substructure
Date: Sun, 13 Nov 2005 11:26:55 -0800 (PST)
Michael Crocker <> wrote:
I assume, due to the method(s) commonly in use, the labs don't get the actual nucleotide sequences from the tests they perform for us, but would like to know, assuming it's possible but not routinely done, would it be prohibitively expensive to get the actual, full sequences between the primers for selected markers? If this were available, then not every one-step-from-modal would necessarily be the same, which should help a lot in discriminating both populations and family lines.
[The type of substructure I would like to see is what is shown on the marker data page at SMGF, for some markers where the repeats occur in distinct blocks.]
We (EA) are the only company at present that is using direct sequencing for Y-SNPs, which means we get to see a string of about 190 nucleotide bases up front and personal. For Y-STR testing you are correct the methodology does not permit examining the string of nucleotide bases. Every so often we do explore them with direct sequencing if we think that perhaps there is a SNP embedded in there - but the cost to do this for every one of the 37 markers would be out of sight - then add in the geneticists time to copy the pherograms to Word and send them along. It is not cost effective and unless there is a proven demand the world will never see such a product. If anyone will develop this, my guess is that it will be DNA-Fingerprint.
Dr. David K.W. Faux
Ethnoancestry USA, Inc.