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Archiver > GENEALOGY-DNA > 2007-12 > 1199157429


From: "RICHARD KENYON" <>
Subject: Re: [DNA] Question for Thomas Krahn - STRs in WOY regions
Date: Mon, 31 Dec 2007 19:17:09 -0800
References: <BAY111-DAV11D291540C53A47C5C4146BC510@phx.gbl>


I don't think what sequencing method is used is important. The problem is whether it's possible to separate out the Y-chromosome from all the other stuff in a typical sample. Perhaps special primers could be designed to extract big chunks of the Y-chromosome. Then each of these chunks could be sequenced, say by a shotgun method, eventually combining these sequences to get the final result. I have no idea whether this would be practical.
Perhaps it's best that we all concentrate on one 50 Kbp segment, as Thomas suggested, then move on to other areas.

Richard R. Kenyon ("Dick")
----- Original Message -----
From: Ron Scott<mailto:>
To: <mailto:>
Sent: Monday, December 31, 2007 5:01 PM
Subject: Re: [DNA] Question for Thomas Krahn - STRs in WOY regions


On Mon, 31 Dec 2007 14:02:29 EST, Ann writes:

> I'm not clear on the technology used by Knome, but I'm not at all sure that
> you can scale things down that way. Some of these new methods chop all the DNA
> in a cell sample into tiny pieces of a few hundred base pairs, sequence those
> short segments, and use computer techniques to align overlapping fragments
> into longer contiguous sequences. If that's what Knome is doing, there's no a
> priori way to say "just give me the Y."

I'm not clear either, but Ann Kiirikki at Knome has told me that this is "something we're wrestling with internally, but higher up ...., " referring to full-Y sequencing and other "limited services." I'm simply suggesting to Knome that there is a huge market of those who would want chr-Y sequencing, if they would offer it. Maybe, one of us could ask Ms. Kiirikki what sequencing method they use.

Ron Scott


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