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Archiver > GENEALOGY-DNA > 2008-01 > 1199193854


From: Vincent Vizachero <>
Subject: Re: [DNA] Question for Thomas Krahn - STRs in WOY regions
Date: Tue, 1 Jan 2008 08:24:14 -0500
References: <BAY111-DAV11D291540C53A47C5C4146BC510@phx.gbl><BAY103-DAV1394D6A53FB52649A029D7C8510@phx.gbl>
In-Reply-To: <BAY103-DAV1394D6A53FB52649A029D7C8510@phx.gbl>


Shotgun sequencing, for all its many advantages, does have real
disadvantages. Separating the Y-chromosome, which Ann mentioned, is
one of them. Shotgun sequencing is also not as accurate as other
methods, which is why huge amounts of overlap are necessary
especially on regions with repeats.

For lurkers interested in this topic, I found this lecture handout
which covers the differences pretty well:

http://www.ibi.vu.nl/teaching/a4g/materials/lect9.pdf

The Wiki entries on the methods are brief, but give enough background
to read the handout.

http://en.wikipedia.org/wiki/Shotgun_sequencing
http://en.wikipedia.org/wiki/Chromosome_walking

I wouldn't suggest that accurate shotgun sequencing of the Y is
impossible, but I do trust Thomas when he suggests that walking
methods are a better bet given the current state of technology.

Vince


On Dec 31, 2007, at 10:17 PM, RICHARD KENYON wrote:

> I don't think what sequencing method is used is important. The
> problem is whether it's possible to separate out the Y-chromosome
> from all the other stuff in a typical sample

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