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Archiver > GENEALOGY-DNA > 2010-02 > 1265939998


From: "Bryan Cook" <>
Subject: Re: [DNA] News item: first ancient human genome sequenced
Date: Thu, 11 Feb 2010 20:59:58 -0500
References: <e4dd.3fa9ee07.38a46414@aol.com>,<COL123-DS63C151F2444DD7C7CC18AC84E0@phx.gbl>,<3271030599658703088@unknownmsgid>,<6a22cf131002110357s3d07aa29sfb8f3c9e060bb7b9@mail.gmail.com><SNT115-W46D09A2D7E2D2EC5512738CC4E0@phx.gbl>
In-Reply-To: <SNT115-W46D09A2D7E2D2EC5512738CC4E0@phx.gbl>


Yes, so it is the roots ( follicles) which are key...I am surprised also
that after so long in storage there is also not a lot of cross
contamination. Bryan

-----Original Message-----
From:
[mailto:] On Behalf Of Steven Bird
Sent: February-11-10 3:43 PM
To:
Subject: Re: [DNA] News item: first ancient human genome sequenced


From:





http://www.enotes.com/forensic-science/hair-analysis





"DNA from cells associated with the root can be extracted and used for DNA
analysis. Analysis of the DNA in the nucleus of the cell can be used for
determining identity and DNA from the y-chromosome focuses on questions of
paternity. Mitochondrial DNA is useful for establishing maternity. In
theory, a single cell contains sufficient DNA to use for DNA analysis and so
a single hair should provide the material required. In practice, a variety
of complications make DNA testing of hair more complex. Roots of hair in the
anogen (growth) phase contain more DNA than hairs from the telogen (resting)
phase. However, hairs in the telogen phase are more likely to fall out
passively. In addition, contamination issues are important as dead skin
cells, which are also shed passively, contain DNA and may be collected from
surfaces along with hair. If a hair from the telogen phase is collected, it
may not contain enough nuclear DNA for analysis, but it might contain
mitochondrial DNA. If the!
hair has been forcibly removed, then pieces of tissue may be attached and
DNA analyses can usually be run easily on these tissue cells."


That's getting to the root of it. :-))





> Date: Thu, 11 Feb 2010 06:57:19 -0500
> From:
> To:
> Subject: Re: [DNA] News item: first ancient human genome sequenced
>
> The article seems to explain otherwise, Bryan.
>
> John Rhodes
>
> On Wed, Feb 10, 2010 at 10:29 PM, Bryan Cook <>
wrote:
>
> > They must have had some hair follicles. I did not think that you could
get
> > DNA from just hair (chitin) and that hair was often contaminated by what
> > people put on it including animal fats. Am I wrong? Bryan
> >
> > -----Original Message-----
> > From:
> > [mailto:] On Behalf Of RICHARD KENYON
> > Sent: February-10-10 8:15 PM
> > To:
> > Subject: Re: [DNA] News item: first ancient human genome sequenced
> >
> > The 11 Feb 2010 issue of Nature contains a very interesting (and
important)
> > article by Rasmussen et al, "Ancient human genome sequence of an extinct
> > Palaeo-Eskimo", pp. 757-762. The DNA from a tuft of hair found in
> > permafrost
> > in Greenland, dated about 4000 bp, is extensively analyzed. They have
> > apparently been able to do a more complete sequencing job of the genome
> > than
> > has been possible for the Neanderthals. They mention, for example,
finding
> > 4024 SNPs on the Y, of which they call 243 to be of "high confindence"
and
> > a
> > total of 353,151 for the what they call the Saqqaq genome. The
haplogroup
> > is
> > stated as Q1a*. They were also able to draw various conclusions about
the
> > phenotype of the Saqqaq man. Interestingly, they don't seem to have had
a
> > problem extracting DNA from hair samples. The supplementary information
> > (only obtainable on-line) is quite extensive, being 87 pages long.
> >
> > Richard R. Kenyon ("Dick")
> > ----- Original Message -----
> > From: <mailto:>
> > To: <mailto:>
> > Sent: Wednesday, February 10, 2010 11:33 AM
> > Subject: [DNA] News item: first ancient human genome sequenced
> >
> >
> > Yup, the whole thing (or pretty much):
> >
> >
> > http://www.the-scientist.com/blog/display/57140/<;
> > http://www.the-scientist.co
> > m/blog/display/57140/>
> >
> > It's the cover article in the February 11 issue of Nature,
> >
> > http://www.nature.com/nature/<http://www.nature.com/nature/>;
> >
> > Only the supplementary material appears to be freely available online
> >
> >
> >
http://www.nature.com/nature/journal/v463/n7282/extref/nature08835-s1.pdf
> > <ht
> > tp://www.nature.com/nature/journal/v463/n7282/extref/nature08835-s1.pdf>
> >
> > Ann Turner
> >
> > -------------------------------
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