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From: steven perkins <>
Subject: [DNA] Article: Microchip electrophoresis of Alu elements for genderdetermination and inference of human ethnic origin.
Date: Sun, 28 Mar 2010 22:31:34 -0400


Sounds like an interesting article on the use of ALU for biogeographic ancestry.

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Electrophoresis. 2010 Mar 22;31(6):981-990. [Epub ahead of print]

Microchip electrophoresis of Alu elements for gender determination and
inference of human ethnic origin.

Njoroge SK, Witek MA, Hupert ML, Soper SA.

Department of Chemistry and Center for BioModular Multi-Scale Systems,
Louisiana State University, Baton Rouge, LA, USA.

We performed a series of multi-locus PCRs followed by the rapid and
efficient microchip electrophoretic sorting of Alu products with LIF
detection. Five polymorphic human-specific Alu insertions (RC5, A1,
PV92, TPA and ACE) were used for inference of human ethnicity and two
monomorphic Alu insertions for sex typing, one fixed on the X
chromosome (AluSTXa) and the other on the Y chromosome (AluSTYa).
These markers were used to generate unique DNA profiles for five
different DNA samples. The PCR-based assays used primers that flank
the insertion point to determine genotypes based on the presence or
absence of the Alu element. A1, RC5, PV92, TPA and ACE were used for
ethnicity determinations and have two alleles, each indicating the
presence (+) or absence (-) of the Alu element on the paired
chromosomes, which results in three genotypes (+/+, +/- or -/-). RC5
and A1 did not show ethnic heterogeneity resulting in a homozygous
(-/-) genotype, which correctly inferred that DNA samples originating
from a Caucasian male and an Asian male were not of African ancestry.
The results from the five Alu markers indicated that these Alu loci
could assist in identifying the individual's ethnicity using microchip
electrophoresis in under 15 min of separation time. Using microchip
electrophoresis and mixed genotype ratios, male DNA-to-female DNA of
1:9, corresponding to a ratio of Y-to-X chromosomes of 1:19, was also
detected for both AluSTXa and AluSTYa to provide gender identification
without requiring separation of female from male cells prior to the
assay.

PMID: 20309932 [PubMed - as supplied by publisher]

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--
Steven C. Perkins
http://stevencperkins.com/
Online Journal of Genetics and Genealogy
http://jgg-online.blogspot.com/
Steven C. Perkins' Genealogy Page
http://stevencperkins.com/genealogy.html
Steven C. Perkins' Genealogy Blog
http://scpgen.blogspot.com/


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