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Archiver > GENEALOGY-DNA > 2011-02 > 1297545301


From: Steven Bird <>
Subject: Re: [DNA] 'The Affair of the Patchytail Zebrafish'
Date: Sat, 12 Feb 2011 15:15:01 -0600
References: <A976DD36A3E44557B1119ACFAC3CEE5C@IanPC>
In-Reply-To: <A976DD36A3E44557B1119ACFAC3CEE5C@IanPC>


Zebrafish are actually quite popular among biologists as an organism for exploring potential genetic disease mechanisms in humans. Plus, they are not nearly as much of a problem when it comes to dissection and tissue extraction as humans would be! ;-)



Thanks for the reference! It appears to be a very interesting study.



Steve



>
> This paper deals with a strain of Zebrafish with a muscular dystrophy; and is a free download on:
> http://hmg.oxfordjournals.org/content/early/2011/02/04/hmg.ddr047.long
>
> and there are pictures of the researchers on, for example:
> http://www.childrenshospital.org/cfapps/research/data_admin/Site1694/mainpageS1694P0.html
>
> So .... what the researchers did was to use typical Mendelian methods to selectively breed
> a population of Zebrafish that couldn't swim well. And, they were found to have a recessive
> trait that when homozygous gives a nasty form of muscular dystrophy - and this clearly has
> its parallels in human disease.
>
> Then .. by using Molecular Biology methods the mutation was identified as occurring
> in the 567th. codon in the dystroglycan gene of Zebrafish's Chromosome 22, where the change
> of a simple 'Valine' (V) to the much more reactive 'Aspartic Acid' (D) is clearly pathological.
>




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